Coding
BPO

Part:BBa_K2461002:Design

Designed by: Jessica Harms   Group: iGEM17_UNebraska-Lincoln   (2017-10-16)


Vanadium Dependent Bromoperoxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 391
    Illegal SapI.rc site found at 115
    Illegal SapI.rc site found at 1312
    Illegal SapI.rc site found at 1720


Design Notes

We had to create silent mutations so that we could remove restriction enzyme sites that were not compatible with the biobrick standards.


Source

This part comes from the paper "Cloning and expression of the gene for a vanadium-dependent bromoperoxidase from a marine macro-alga, Corallina pilulifera" The link to the paper can be found here. http://www.sciencedirect.com/science/article/pii/S0014579398005006

References